Table I.
Relative esterification rate of strains used in this study
| Strain | Exogenous sterol |
Growth temperature |
Relative esterification ratea |
|---|---|---|---|
| °C | % | ||
| upc2-1 | cholesterol | 23 | 0.94 ± 0.23 |
| upc2-1 oshΔ osh4-1 | cholesterol | 23 | 0.37 ± 0.042 |
| upc2-1 oshΔ OSH4 | cholesterol | 23 | 0.37 ± 0.072 |
| upc2-1 | cholesterol | 37 | 1.9 ± 0.27 |
| upc2-1 oshΔ osh4-1 | cholesterol | 37 | 0.27 ± 0.064 |
| upc2-1 oshΔ OSH4 | cholesterol | 37 | 0.44 ± 0.10 |
| upc2-1 | ergosterol | 37 | 0.39 ± 0.067 |
| upc2-1 oshΔ osh4-1 | ergosterol | 37 | N.D. |
| upc2-1 oshΔ OSH4 | ergosterol | 37 | 0.042 ± 0.003 |
| upc2-1 | cholesterol | 37 | 2.1 ± 0.24 |
| upc2-1 pik1-83 | cholesterol | 37 | 0.64 ± 0.057 |
| upc2-1 stt4-4 | cholesterol | 37 | 0.63 ± 0.090 |
| upc2-1 mss4-2ts | cholesterol | 37 | 0.23 ± 0.017 |
| upc2-1 | cholesterol | 30 | 2.3 ± 0.25 |
| upc2-1 osh1Δ | cholesterol | 30 | 2.5 ± 0.046 |
| upc2-1 osh2Δ | cholesterol | 30 | 2.6 ± 0.098 |
| upc2-1 osh3Δ | cholesterol | 30 | 1.5 ± 0.11 |
| upc2-1 osh4Δ | cholesterol | 30 | 2.4 ± 0.19 |
| upc2-1 osh5Δ | cholesterol | 30 | 1.8 ± 0.25 |
| upc2-1 osh6Δ | cholesterol | 30 | 2.1 ± 0.091 |
| upc2-1 osh7Δ | cholesterol | 30 | 2.2 ± 0.10 |
| upc2-1 osh3Δ osh5Δ | cholesterol | 30 | 1.2 ± 0.12 |
N.D., no ergosteryl ester detected.
a
Esterification rate, percentage of total sterol taken up that is esterified per minute.