Figure 2.
Time-lapse microscopy of perforin-induced death in MS9II cells. MS9II cells were followed by time-lapse microscopy. (A) Images of morphology, annexin V, and PI were taken every 20 s. 80 nM perforin was added after the fifth frame (1 min 22 s). Plasma membrane protrusions are indicated by white arrows. Black arrow indicates the time of perforin addition. Asterisk indicates the cell analyzed in B. The full video is presented in Video 2 (available at http://www.jcb.org/cgi/content/full/jcb.200510072/DC1). (B) The fluorescence of annexin V and PI in the cell indicated by an asterisk in A was plotted as relative fluorescence compared with maximum fluorescence for that fluorochrome. Perforin addition is noted by the dashed arrow below the x axis. Arrows indicate the points at which the frames in A were recorded.