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. 2006 Apr 10;173(1):133–144. doi: 10.1083/jcb.200510072

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — CTL-induced death of MS9II cells. Allogenic CTLs (H-2^b anti–H-2^k) were generated in a one-way mixed lymphocyte reaction from wild-type (WT), granzyme AB^−/−, and perforin^−/− mice. (A) MS9II cells preloaded with 100 μCi ⁵¹Cr were treated with CTLs from wild-type, granzyme AB^−/−, and perforin^−/− mice at the effector to target ratios indicated. The percentage of ⁵¹Cr released was assayed at 4 h. The data points shown are the means ± SD of data pooled from three independent assays each prepared in triplicate. (B) Real-time PCR for perforin and granzyme A, B, C, and K was performed on cDNA transcribed from mRNA isolated from WT and granzyme AB^−/− mice. Each cDNA sample was assayed in duplicate. Data presented is the mean fold increase in message (calculated from the means of duplicate samples) relative to the level of granzyme C in naive T cells from wild-type mice. Data represents message amplified from CTLs of three mice tested independently in each case ± SD (error bars).