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. 2006 Apr 24;173(2):173–179. doi: 10.1083/jcb.200601075

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Localization of Kif2a in control and p150_CC1-injected PtK1 cells. Control bipolar spindle showing Kif2a (A) and tubulin (A′) localization. (B) Cells injected with p150_CC1 and rhodamine-tubulin were then fixed and stained for Kif2a. Tubulin (B′) is from the injected rhodamine-tubulin signal. (C) Cells treated with 200 μM monastrol and injected with p150_CC1 and tubulin showing the localization of Kif2a (C) and tubulin (C′) in the monopolar spindle. Images are the addition of two planes of a z series in which each pole is most in focus. Asterisks denote the position of the centrosome as found in other planes of the z series. Bar, 5 μm.