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. 2006 Apr 24;173(2):253–264. doi: 10.1083/jcb.200508196

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — The CCGTTA hexamer is necessary and sufficient for Ovol1 recognition. (A) Sequence of oligonucleotides used in EMSA assays. (B) Competition assays were performed with Ovol1D (an oligonucleotide containing a high-affinity Ovol1-binding site) as the labeled probe and Ovol1D or oligonucleotides with indicated point mutations within the CCGTTA sequence as unlabeled competitors (present at a 100-fold excess). (C) EMSA assays using the Ovol1D oligonucleotide or its mutant derivatives showing that the CCGTTA hexamer sequence was necessary for Ovol1–DNA interaction. (D) EMSA assays using the nso oligonucleotide or its derivative showing that the CCGTTA hexamer sequence was sufficient for Ovol1–DNA interaction. Ovals and arrowheads indicate the positions of Ovol1–DNA complexes and free probes, respectively.