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. 2006 May 8;173(3):417–429. doi: 10.1083/jcb.200508121

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Co-localization and interaction of endogenous β-catenin and Fat1 in VSMCs. (A) Immunofluorescence analysis of β-catenin (β-cat, red), Fat1 (green), and areas of colocalization (merge, yellow). Nuclei were stained with DAPI (blue), as indicated. β-Catenin and Fat1 colocalization at cell–cell junctions is indicated by an arrowhead. Bar (10 μm) applies to all panels. (B) Detail from panels in A, showing staining for Fat1 (green), but not β-catenin (red), at the cellular free edge. Bar, 10 μm. (C) Co-immunoprecipitation of endogenous β-catenin and Fat1. Cell lysates were incubated with antibodies specific for Fat1 (top) or β-catenin (bottom) or normal rabbit or mouse IgG, and the immunoprecipitated complexes were analyzed by Western blot for Fat1 and β-catenin, as indicated.