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. 2006 May 22;173(4):545–557. doi: 10.1083/jcb.200601067

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Milton recruits endogenous KHC to mitochondria. COS7 cells were immunostained with anti-KHC (white) and anti-milton (green), and mitochondria were labeled with MitoTracker orange (pink). (A and B) Control cells differed in the distribution of endogenous KHC. It was enriched on mitochondria in some (A), and had a more cytoplasmic distribution in others (B). (C) In contrast, in cells transfected with milton, endogenous KHC always colocalized with mitochondria, and little or no KHC could be detected elsewhere. Bar, 20 μm.