Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 May 22;173(4):587–589. doi: 10.1083/jcb.200509075

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Rac activation regulates adhesion turnover. (a) CHO-K1 cells coexpressing S273D-paxillin-GFP and a PIX mutant, which lacks nucleotide exchange activity (PIX-LL) showed large adhesions (arrows) that disassembled slowly (Table III). Bar, 5 μm. (b, top) CHO-K1 cells coexpressing dominant-negative (N17-Rac) and S273D-paxillin-GFP show large and stable adhesions (arrows) that disassemble slowly (Table IV). (bottom) CHO-K1 cells coexpressing CA- (V12-Rac) and WT-paxillin-GFP exhibit numerous small but stable adhesions (Table IV) at the cell periphery (arrows). Bar, 5 μm. (c) CHO-K1 cells coexpressing the Rac GEF Tiam1 and WT-paxillin-GFP show numerous small paxillin-containing adhesions (arrows) that disassemble very rapidly (Table IV). Bar, 5 μm. (d) S273-paxillin phosphorylation increases Rac activation. Rac activity from CHO-K1 lysates coexpressing FLAG-WT-Rac and paxillin-GFP mutants was measured using a GST-PBD pull-down assay. CHO-K1 lysates coexpressing FLAG-V12-Rac and WT-paxillin-GFP served as a positive control. (top) Active Rac in GST-PBD bead pellets was detected by immunoblotting using an anti-FLAG antibody. (bottom) Equal protein aliquots of lysates served as loading controls. Rac activation increased eightfold with S273D-paxillin when compared with S273A-paxillin and 1.5-fold compared with WT-paxillin.