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. 2006 Jun 5;173(5):651–658. doi: 10.1083/jcb.200603092

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Identification of UPS1. (A) A model for alternative topogenesis of Mgm1p at the IM. The TIM23 translocon (gray), TM1 (blue), and TM2 (yellow) of Mgm1p, the mitochondrial processing peptidase (MPP, green) and Pcp1 protease (green) are shown. (B) WT cells were labeled with [³⁵S]-methionine and -cysteine, and chased for the indicated time period. Proteins were examined by immunoprecipitation using anti-Mgm1p antibodies. (C) Cell lysates from WT and indicated mutant strains grown in YPGalSuc were analyzed by immunoblotting using anti-Mgm1p antibodies. (D) Isolated mitochondria were analyzed by immunoblotting using the indicated antibodies.