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. 2006 Jun 5;173(5):755–765. doi: 10.1083/jcb.200512141

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Effects of exogenously expressed proteins on Ca²⁺ oscillation frequency in mGluR5a-expressing HeLa cells. (A) Emission ratio change of Indo-1 signals in cells stimulated with 100 μM of glutamate (horizontal bars). (B) Histograms of Ca²⁺ oscillation frequency in mGluR5a-expressing cells stimulated with 100 μM of glutamate. (C) Western blot analysis of cell lysates prepared from HeLa cells transfected with mGluR5a alone (lane 2), mGluR5a plus C/V-PHD (lane 3), mGluR5a plus IRIS-1 (lane 4), or mGluR5a plus IRIS-1–Dmut (lane 5). Nontransfected cells were used as a control (lane 1). An anti-mGluR5 antibody was used. Molecular mass markers are shown on the left (×10⁻³).