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. 2006 Jun 19;173(6):853–859. doi: 10.1083/jcb.200602046

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Opposing effects of PDI and ERp72 on the conformation of CT. (A) His-tagged ERp72 protein was purified from bacteria and analyzed by SDS-PAGE followed by Coomassie staining or immunoblotting with an antibody against ERp72. (B) CTA was incubated with BSA, PDI, or ERp72 followed by the addition of trypsin. Samples were subjected to SDS-PAGE followed by immunoblotting with an anti-CTA antibody.