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. 2006 Jun 19;173(6):917–926. doi: 10.1083/jcb.200510010

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Colocalization of Rab5/9 with EEA1. (A) Colocalization of CFP-Rab5/9 with the early endosome marker EEA1 in fixed HeLa cells stained with anti-EEA1 and anti-GFP antibodies. The square in A is enlarged for both markers (right); arrows are included to facilitate comparison. Bar, 10 μm. (B) Quantitation of CFP-Rab5/9, CFP-Rab5, and YFP-Rab9 colocalization. Venn diagrams are shown as follows: CFP-Rab5/9, CFP-Rab5, and YFP-Rab9 versus EEA1; CFP-Rab5/9 and CFP-Rab5 versus endocytosed anti–CI-MPR IgG; and CFP-Rab5/9 and CFP-Rab5 versus YFP-Rab5. The number of positive structures is indicated below each protein name; overlap is indicated as described in the Results section.