Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jan 15;176(2):155–162. doi: 10.1083/jcb.200607178

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Monomethylated H4K20 is strongly reduced in PR-Set7 brains. (a) Wild-type (WT) and PR-Set7/PR-Set7 third-instar larval brains were stained with Hoechst. The two strongly staining rings (dense nuclei) observed in wild type are the optic lobes. (b) Western blots of extracts from wild-type and PR-Set7/PR-Set7 third-instar larval brains probed with anti–mono-, anti–di-, and anti–trimethylated H4K20 (mono-, di-, and tri-me), anti–histone H4, and anti-lamin antibodies. The intensity of the bands was quantified by ImageJ, and the value of mono-, di-, or trimethylated H4K20 was normalized to the values of both histone H4 and lamin (see Table S1, available at http://www.jcb.org/cgi/content/full/jcb.200607178/DC1). The graph shows the ratio of PR-Set7/PR-Set7 mutant to wild-type values. Error bars show two SDs (n = 3). (c) Neuroblasts were costained with anti–monomethylated H4K20 (mono-me; red) and anti-PH3 antibodies (green). DNA was stained with Hoechst (blue). (d) Monomethylated H4K20 (red) is distributed all along the chromosomes. Bars: (a) 100 μm; (c and d) 5 μm.