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. 2007 Jan 15;176(2):155–162. doi: 10.1083/jcb.200607178

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Cyclin B is down-regulated in PR-Set7 by APC/C-dependent proteolysis. (a) Western blots of extracts from wild-type (WT), homozygous PR-Set7, and double-homozygous PR-Set7,cdc27 third-instar larval brains were probed with anti–cyclin B, anti–cyclin A, and anti-lamin antibodies. (b) Cyclin A and cyclin B mRNA levels in wild type and PR-Set7/PR-Set7 were measured by quantitative real-time PCR and normalized to the level of rp-49 mRNA. Total RNA was isolated twice independently, and each RNA was measured twice at different dilutions. The graph shows the ratio of PR-Set7 mutant values to wild type. Error bars show two SDs (n = 4).