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. 2007 Jan 15;176(2):155–162. doi: 10.1083/jcb.200607178

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — The DNA damage checkpoint is activated in PR-Set7. (a) S phase indexes of wild type (WT), PR-Set7/PR-Set7, mei-41^D3/mei-41^D3; PR-Set7/PR-Set7, and mei-41^D3/mei-41^D3 (see Table S4, available at http://www.jcb.org/cgi/content/full/jcb.200607178/DC1). (b) Mitotic indexes of wild type, PR-Set7/PR-Set7, mei-41^D3/mei-41^D3;PR-Set7/PR-Set7, and mei-41^D3/mei-41^D3 (Table S2). (c) Western blots of extracts from wild-type, PR-Set7/PR-Set7, and mei-41^D3/mei-41^D3;PR-Set7/PR-Set7 third-instar larval brains were probed with anti–cyclin B and anti-lamin antibodies. (d) Western blots of extracts from wild-type, PR-Set7/PR-Set7, mei-41^D5/mei-41^D5;PR-Set7/PR-Set7, and grp^fsA4/grp^fsA4;PR-Set7/PR-Set7 third-instar larval brains were probed with anti–cyclin B, anti-lamin and anti–monomethylated H4K20 (mono-me) antibodies. Two different extracts from mei-41^D5/mei-41^D5;PR-Set7/PR-Set7 were used.