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. 2007 Jan 15;176(2):183–195. doi: 10.1083/jcb.200610099

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Loss of APC leads to polyploidy. (A) Control or APC-targeting siRNA-transfected U2OS cells were treated with 1.25 μg/ml taxol or nocodazole for 20 h or were left untreated before harvesting and staining with FITC-labeled anti–cyclin B1 antibody and 7AAD for DNA content as in Fig. 3. The proportion of polyploid cells (>4n DNA; indicated as blue shaded area in Fig 3 A) in APC-positive (iContr) and APC-deficient (iAPC) cultures was determined. Graph represents the percentage of polyploid cells as the mean ± SD (error bars). (B) Accumulation of polyploid (>4n DNA) cells is accelerated in the absence of APC. U2OS cells were transfected with APC or control targeted siRNA, arrested, and stained as in A. The ratio of the polyploid population measured as in A to the tetraploid G1 population measured as in Fig. 3 was calculated. The graph represents the mean of polyploid/tetraploid G1 ratios ± SD for APC-deficient (iAPC) and control (iContr) U2OS cells, which were either unsynchronized or treated with mitotic poisons as indicated. (A and B) Data show a representative example of one out at least three independent experiments; for each experiment, duplicate samples were collected for flow cytometry analysis.