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. 2007 Jan 15;176(2):223–230. doi: 10.1083/jcb.200606116

Figure 4.

Figure 4.

The N-terminal phosphorylation state of Had/Na+,K+ ATPase does not affect membrane association. (A) Western blot of 32 hpf embryonic extracts shows that HisMyc∷Hadwt and HisMyc∷Had3A are enriched within the membrane and cytoskeletal fraction. (B and C) Confocal fluorescence microscopic images. Myocardial wt cells expressing HisMyc∷Hadwt (B and B′) and HisMyc∷Had3A (C and C′). Both recombinant forms of the Na pump are mostly localized at the outer cell membrane.