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. 2007 Jan 15;176(2):231–241. doi: 10.1083/jcb.200606027

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — The nuclear membrane vesiculates after activation. Ultrathin cryosections of neutrophils stimulated with 20 nM PMA for 60 (a) and 180 (d) min and labeled with an antibody against the nuclear envelope–specific lamin B receptor. Bound antibody was detected with a gold-conjugated secondary antibody. In 60-min–stimulated neutrophils, the lamin B receptor was found on the inner nuclear membrane, as expected (a, arrows). 180 min after stimulation, the lamin B receptor was found in vesicles (d, arrows) in the cytoplasm. Nuclear material (d, arrowheads) is decondensed and not enclosed by a membrane. (b) 60 min after activation, neutrophils have flattened out and exhibit a lobulated nucleus and numerous granules. (c) Immunofluorescence staining for the nuclear membrane delineates the nuclear lobules (green) and is in continuity with the endoplasmic reticulum (red). (e and f) After 180 min of stimulation, neutrophil nuclei are inflated and fill nearly the entire cell. Some remaining granules are found in the periphery of the cell, while remnants of the nuclear membrane and the endoplasmic reticulum are restricted to a small central area surrounded by nuclear material (arrows). Bars: (a and d) 500 nm; (b, c, e, and d) 10 μm.