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. 2007 Jan 29;176(3):343–353. doi: 10.1083/jcb.200606023

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — PIPKIγ targets to AJs by a direct interaction with E-cadherin. (A) PIPKIγ targets to AJs in both MDCK and MCF10A cells. Horizontal (x-y) and vertical (x-z) sections show colocalization of PIPKγ and E-cadherin (ECD). Arrows show the vesicular compartments where PIPKIγ and E-cadherin colocalize. Bar, 10 μm. (B) PIPKIγ associates with cadherin complexes. Immunoprecipitations (IP) and immunoblots (IB) were performed as indicated. NCD, N-cadherin; VECD, VE-cadherin. Normal mouse (mIgG) or rabbit IgG (rIgG) were used as controls. (C) PIPKIγ directly and preferentially interacts with the dimerized cytoplasmic domain of E-cadherin. A GST pull-down assay was performed using 1 μg of each purified protein. (D) PIPKIγ preferentially binds the E-cadherin dimmer in vivo. Myc-tagged ECDT or HR-ECDT was overexpressed in HEK293 cells and immunoprecipitated.