Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Feb 26;176(5):573–580. doi: 10.1083/jcb.200612043

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Knockdown of MIIA or MIIB differentially alters cell polarity. (A) CHO.K1 cells were transfected with pSUPER-GFP vector or pSUPER-GFP-RNAi against MIIA or MIIB, and blotted for MIIA or MIIB. GIT1 is a loading control. (B) Representative images of MIIA- (top) and MIIB-depleted cells (bottom) stained for MIIA or MIIB, respectively. Arrows point to transfected cells. (C–E) Time-lapse series of MIIA-depleted (C; Video 1), MIIB-depleted (D; Video 2), or control cells (E; Video 3). In C, arrowheads point to the defect in tail retraction. (D) Arrows point to transfected, unpolarized cells. Videos are representative of >25 cells in 6 independent experiments. (F) Fluorescence images depicting the enlargement of MIIB-deficient cells. Images are representative of >300 cells. (G; top) Effect of MIIB knockdown on cell area. Data are the mean ± the SD of 4 independent experiments comprising >300 cells/experiment. (bottom) Cell areas in control and MIIB-depleted cells. (H) MIIB-deficient cell showing clockwise rotation of the nucleus. Arrowhead points to nucleolus; top-right indicator, clockwise angular displacement (Video 4). (I) Depolarization of the Golgi is observed in MIIB-deficient cells (arrows) and not in nontransfected cells (arrowheads). Bars: (C) 40 μm; (D–F and H) 50 μm. Videos 1–4 are available at http://www.jcb.org/cgi/content/full/jcb.200612043/DC1.