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. 2007 Feb 26;176(5):573–580. doi: 10.1083/jcb.200612043

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Contractility-deficient mutants of MIIA and MIIB exhibit differential rescue of actin bundling, protrusion, and adhesion dynamics in MIIA- and MIIB-deficient cells. Inhibited FRAP of GFP-MIIA N93K (A) and GFP-MIIB R709C (B) in actin bundles. Data are the mean ± the SD of >20 individual measurements from four independent experiments. (C) Differential FRAP of GFP-MIIA-WT and GFP-MIIB-WT in actin bundles. (D) A MIIB-depleted cell cotransfected with paxillin-GFP and mChe-MIIB R709C (not depicted). (E) Localization of GFP-MIIB and GFP-MIIB R709C. Arrows show the direction of migration. (F) Polarity index of migrating CHO.K1 cells. Data are the mean ± the SD of >100 cells analyzed/condition. (G) Time-lapse series of a MIIA-depleted cell expressing paxillin-GFP and mChe-MIIA (not depicted). Bars: (D) 3 μm; (G) 15 μm.