Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Feb 26;176(5):709–718. doi: 10.1083/jcb.200610046

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Altered osteoblast differentiation in calvarial cells from TgMek-dn and -sp mice. Cells were isolated from calvaria of newborn wild-type and transgenic animals. (a) Time course of transgene expression. Cells were plated and grown in differentiating medium for the indicated times before measurement of transgene mRNA by RT-PCR. (b) Mek-dn and -sp transgene expression alters ERK phosphorylation. Cells were grown as in a and harvested after 10 d for measurement of total and phospho-ERK by Western blotting. The indicated groups were treated with the Raf inhibitor ZM336372 2 h before harvest. (c) Transgene expression does not alter cell growth. (d–j) MEK-DN inhibits, whereas MEK-SP stimulates, osteoblast differentiation. The following differentiation markers were measured: mineralized nodules in 14-d cultures (d), alkaline phosphatase activity (e), total cell layer–associated, acid-extractable calcium (f), and OCN, BSP, ALP, and Runx2 mRNA levels (g–j; all measured by real-time RT-PCR). Values are the mean ± the SD of triplicate independent samples.