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. 2007 Feb 26;176(5):719–727. doi: 10.1083/jcb.200609008

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Matrix-specific PAK activation stimulates permeability in response to multiple atherogenic stimuli. (A) Endothelial cells plated on transwell membranes coated with either MG or FN were stimulated with 50 ng/ml MCP-1, 10 ng/ml TNFα, or 100 μg/ml oxLDL for 2 h, and the HRP leak across the membrane was assessed. Values are means ± SD (n = 3–4). (B) BAE cells plated on FN-coated transwell membranes were treated with either a control or a PAK-Nck inhibitory peptide (20 μg/ml for 1 h). Monolayers were stimulated with MCP-1, TNFα, or oxLDL for 2 h, and the HRP leak across the membrane was assessed. Values are means ± SD (n = 3). (C) Endothelial cells plated on FN-coated transwell membranes were transfected with HA-tagged PAK AID. After 24 h, monolayers were stimulated with MCP-1, TNFα, or oxLDL for 2 h, and the HRP leak across the membrane was assessed. Values are means ± SD (n = 3). Transfection efficiency ranged from ∼35 to ∼50% as determined by immunocytochemistry.