Table 2.
Immunogenic (in vivo expressed) exoproteins of MW2 and LAC.
| Proteina | MW2 | LAC | Immunoreactivity |
|---|---|---|---|
| AhpC, alkyl hydroperoxide reductase | ME, S | ME, S | NI, I |
| AroA, chorismate mutase | – | S | I |
| Asp23, alkaline shock protein 23 | – | S | NI, I |
| Atl, autolysin | ME, S | ME, S | I |
| ClpP, ATP-dependent Clp protease | S | S | NI, I |
| Cna, collagen adhesin precursor | ME, S | – | I |
| Coa, coagulase | ME | – | NI, I |
| DeoD, purine nucleoside phosphorylase | ME | ME | NI, I |
| Ear | S | S | I |
| Eno, enolase | S | S | NI, I |
| Fba, fructose bisphosphate aldolase | S | – | NI, I |
| Fhs, formate tetrahydrofolate ligase | ME, S | ME, S | I |
| Ftn, ferritin | – | S | I |
| Gap, glyceraldehyde 3-phosphate dehydrogenase | ME, S | ME, S | I |
| GlyA, serine hydroxymethyltransferase | – | S | I |
| Gpi, glucose 6-phosphate isomerase | ME | ME | NI, I |
| GuaB, inositol-monophosphate dehydrogenase | ME | – | NI, I |
| Idh1, isocitrate dehydrogenase | – | S | NI, I |
| Lip, lipase | S | S | I |
| MW0525, hexulose 6-phosphate synthase | – | S | I |
| MW0896, 2′−5′ RNA ligase | – | S | NI, I |
| MW1795, hypothetical protein | – | S | NI, I |
| MW1870, thioredoxin | – | S | NI, I |
| PdhA, pyruvate dehydrogenase subunit A | ME | ME | NI, I |
| PdhB, pyruvate dehydrogenase subunit B | S | S | NI, I |
| Pgd, phosphogluconate dehydrogenase | ME | ME | NI, I |
| Pgi, phosphoglucose isomerase | S | S | NI, I |
| Plc, 1-phosphatidylinositol phosphodiesterase | S | S | I |
| Pta, phosphate acetyltransferase | – | S | I |
| RocD, ornithine aminotransferase | S | – | NI, I |
| Sak, staphylokinase | ME | ME, S | I |
| Sec3, staphylococcal enterotoxin C3 | S | – | I |
| Seq, staphylococcal enterotoxin Q | ME | ME | I |
| SodA, superoxide dismutase | S | S | I |
| Spa, immunoglobulin-binding protein A | ME, S | ME, S | NI, I |
| SspB, cysteine protease precursor | ME, S | ME, S | I |
| Sulfatase | S | – | NI, I |
| Tkt, transketolase | S | – | I |
| TpiA, triosephosphate isomerase | – | ME | I |
| Tsf, translation elongation factor Ts | ME | S | I |
| Tuf, translation elongation factor Tu | ME, S | ME, S | NI, I |
| Unknown 1 | ME | – | I |
| Unknown 2 | ME | – | I |
| Unknown 3 | – | ME | I |
| Unknown 4 | – | ME | I |
| Unknown 5 | – | ME | I |
| Unknown 6 | – | ME | I |
Protein identities were obtained by overlay analysis as described under Experimental procedures. Results are representative of three experiments using serum pooled from 10 to 15 mice.
ME, mid-exponential phase of growth; S, stationary phase of growth; NI, non-immune sera; I, immune sera.