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. 2007 Mar 12;176(6):757–763. doi: 10.1083/jcb.200701065

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — KNL-2 is required for CeCENP-A but not histone H3 targeting to chromatin. (A) CeCENP-A and KNL-2 localization in wild-type, KNL-2–depleted, or CENP-A– depleted embryos. (B) KNL-2 depletion does not prevent histone H3 localization. Note that GFP-histone H2b levels on chromatin were also unaffected (Fig. 1 A). (C) Quantitation of CeCENP-A and histone H3 localization. For CeCENP-A analysis, KNL-2 was depleted in embryos expressing GFP-histone H2b, and the fluorescence intensity ratio of CeCENP-A to GFP-histone H2b was calculated in deconvolved images of fixed embryos. For histone H3, the signal on chromatin was directly quantified. Error bars represent the SEM with a confidence interval of 0.95. (D) KNL-2 depletion does not affect CeCENP-A protein levels. Immunoblot comparing serially diluted control C. elegans extract to CeCENP-A–and KNL-2–depleted C. elegans extracts is shown. α-Tubulin was used as a loading control. Bars, 5 μm.