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. 2007 Mar 12;176(6):765–770. doi: 10.1083/jcb.200610043

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Spindle length determination is highly dynamic in Xenopus extracts. (A) Spindles assembled in X. tropicalis extracts containing X-rhodamine tubulin were diluted with three volumes of X. laevis or X. tropicalis extract supplemented with Alexa Fluor 488 tubulin, and fixed for examination at various time points. Quantification of spindle length over time after mixing revealed steady-state spindle lengths were reached within ∼5 min, and they corresponded to those in the premixed control of 75% X. laevis 25% X. tropicalis extract. (B) The same experiment described in A, except that the spindles were assembled in X. laevis extract containing Alexa Fluor 488 tubulin, and diluted with X. tropicalis or X. laevis extract containing X-rhodamine tubulin. Quantification of spindle length over time after mixing is shown. Error bars are the SDs. Bars, 10 μm.