Figure 4.
Nascent CENP-A loads exclusively during G1. (A) Schematic of cell synchronization and late G1 labeling protocol. (B) Representative images at indicated time points. Percentages of cells that have loaded CENP-A are indicated below. Note that the 11% of cells that have loaded CENP-A by 9.5 h represent cells that have already entered the next G1 phase, as evident from the absence of CENP-A loading in cells blocked in mitosis by nocodazole.