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. 2007 Mar 12;176(6):831–841. doi: 10.1083/jcb.200610053

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Thin, but not thick, filament formation is compromised in E33 mutant IFMs. (A) RT-PCR analysis of IFM-specific actin (Act88F) expression; transcripts were not detectable in E33 mutants, whereas expression was normal in the wild-type (WT) and the parental Smn^E alleles. Troponin I (TnI) and tropomyosin 2 (Tm2) RT-PCR products served as internal controls. (B) Northern blot of total thoracic RNA from adult wild-type (WT) or E33 mutants shows that steady-state U1 and U2 snRNA levels are normal. U3 and U6 snRNA levels used as loading controls. 2 μg of total RNA were loaded in each lane. (C–E) Loss of actin expression was confirmed in E33 mutant myofibers using phalloidin. Whereas the characteristic repetitive blocks of actin were observed in wild-type (WT) myofibrils (c), no staining was detected in the mutant (d). Actin staining in the E33 mutants was restored by transgenic expression of YFP-dSMN (E). Molecular weight markers in base pairs.