Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Apr 9;177(1):29–37. doi: 10.1083/jcb.200611079

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Oligosaccharide donor competition assays. OST activity was assayed using a constant concentration of the acceptor tripeptide (5 μM in A, B, and F and 10 μM in C–E). (A) Glycopeptide products from assays of the T. vaginalis (a and c) or S. cerevisiae (b) OST using 1 μM G₃M₉GN₂-PP-Dol (a) or 0.6 μM G₃M₉GN₂-PP-Dol plus 1.5 μM M₅GN₂-PP-Dol (b and c) were resolved by HPLC. For clarity, column profiles have been offset on the vertical axis. (B) The T. vaginalis (squares) or S. cerevisiae (circles) OST were assayed using 1.5 μM M₅GN₂-PP-Dol and increasing concentrations of G₃M₉GN₂-PP-Dol. Glycopeptide products were resolved by HPLC to determine the percentage of M₅GN₂-NYT. The dashed line indicates the composition (in percentage of M₅GN₂-PP-Dol) of the donor substrate mixtures. (C–E) Purified S. cerevisiae (Sc) or detergent extracts of C. neoformans (Cn), E. histolytica (Eh), T. vaginalis (Tv), or T. cruzi (Tc) membranes were assayed using the following donor substrate mixtures: 1 μM G₃M₉GN₂-PP-Dol + 1 μM M₅GN₂-PP-Dol (C), 1 μM G₃M₉GN₂-PP-Dol + 1 μM M₉GN₂-PP-Dol (D), and 1 μM M₉GN₂-PP-Dol + 1 μM M₅GN₂-PP-Dol (E). Glycopeptides were resolved by HPLC to determine product composition. (F) The S. cerevisiae and T. vaginalis OST were assayed using the following mixture: (G₂M₉GN₂-PP-Dol/G₁M₉GN₂-PP-Dol/M₉GN₂-PP-Dol/M₅GN₂-PP-Dol, 35:6:2:57). Glycopeptides were resolved by HPLC (top). The M₅GN₂-NYT (M5), G₁M₉GN₂-NYT (G1), and G₂M₉GN₂-NYT (G2) peaks are labeled. The T. vaginalis OST (closed bars), but not the yeast OST (open bars), shows reduced utilization of G₂M₉GN₂-PP-Dol and G₁M₉GN₂-PP-Dol relative to M₅GN₂-PP-Dol (bottom).