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. 2007 Apr 9;177(1):29–37. doi: 10.1083/jcb.200611079

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Kinetic parameters for the oligosaccharide donor substrate. OST activity was assayed using a constant concentration of the acceptor tripeptide substrate (10 μM in A, B, and D and 15 μM in C) and variable concentrations of M₅GN₂-PP-Dol (A–C) or M₉GN₂-PP-Dol (D). (A, C, and D) Lineweaver-Burk plots (1/OST activity versus 1/[OS-PP-Dol]) for the T. vaginalis (A; K_m = 0.22 μM), E. histolytica (C; K_m = 0.72 μM), or T. cruzi (D; K_m = 0.49 μM) OST were linear. (B) An Eadie-Hofstee plot (OST activity vs. OST activity/[OS-PP-Dol]) for the T. vaginalis (K_m = 0.19 μM) OST was linear. The inset shows an Eadie-Hofstee plot for the S. cerevisiae OST using M₅GN₂-PP-Dol as the donor substrate.