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. 2007 Apr 9;177(1):39–49. doi: 10.1083/jcb.200608066

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Copyright © 2007, The Rockefeller University Press

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Figure 9. — Desumoylation of Cdc3-HA after mitosis is delayed in a nup53 mutant. WT cells or a strain containing the nup53-Δ405-430 mutant and expressing CDC3-HA were arrested in M phase with nocodazole (Noc). Cells were then released from arrest and allowed to exit mitosis. At the indicated times, Cdc3-HA sumoylation and Clb2p degradation were evaluated by Western blotting of whole-cells lysates using anti-HA and anti-Clb2p antibodies.