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. 2007 Apr 9;177(1):51–61. doi: 10.1083/jcb.200611036

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Tpg induces Herp redistribution. (A) Endogenous Herp was visualized by immunofluorescence microscopy after incubation of HeLa cells for 1 h with either 2 μM Tpg or DMSO solvent. (B) Kinetic analysis of Herp induction and redistribution. (top) Endogenous Herp was detected by Western blot analysis of extracts from HeLa cells treated with Tpg for the indicated time periods. (bottom) HeLa cells transiently expressing Herp-RFP fusion protein were visualized at 5-min intervals (until 1 h) after the addition of Tpg. The images taken at the 15-min intervals are presented. (C, top) Immunofluorescence of Herp in cells incubated with either DMSO or Tpg in the absence (left) or presence (right) of 50 μM CHX. (bottom) CHX inhibits the stabilization of UbG76V-GFP by MG132. Cells transiently expressing UbG76V-GFP were incubated for 1 h with the indicated reagents and were subsequently visualized by fluorescence microscopy. Bars, 50 μm.