Table I.
List of antibodies, suppliers, and dilutions used
| Antigen | Clone | Isotype | Company | Dilution | Fixation |
|---|---|---|---|---|---|
| 1B10 | 1B10 | IgM | Sigma-Aldrich | 1:50 | * |
| BCA-225 | CU-18 | IgG1 | Signet | 1:10 | M |
| Bcl-2 | 124 | IgG1 | DakoCytomation | 1:75 | M |
| Bcl-2 | 8C8 | IgG1 | Neomarkers | 1:50 | M |
| CALLA | 56C6 | IgG1 | Novocastra | 1:50 | M |
| CD31 | JC70A | IgG1 | DakoCytomation | 1:50 | * |
| CD34 | QBEnd/10 | IgG1 | Novocastra | 1:50 | * |
| CD45 | Bra-55 | IgG1 | Oncogene Research Products | 1:250 | * |
| CD49f | GoH3 | IgG2a (rat) | BD Biosciences | 1:500 | ** |
| CDw75 | LN1 | IgM | Neomarkers | 1:30–1:50 | M |
| Chondroitin sulphate | 9.2.27 | IgG2a | BD Biosciences | 1:50 | M/F |
| Collagen IV | 1042 | IgG2b | Monosan | 1:5 | M |
| E-cadherin | HECD-1 | IgG1 | Zymed Laboratories | 1:25 | M |
| EMA | E29 | IgG2a | DakoCytomation | 1:10 | M |
| EpCAM | Ber-EP4 | IgG1 | DakoCytomation | 1:100 | ** |
| EpCAM | VU1D9 | IgG1 | Novocastra | 1:25–1:100 | M** |
| EpCAM-FITC | VU1D9 | IgG1 | Abcam | 1:100 | ** |
| Integrin-β4 | 3E1 | IgG1 | Chemicon | 1:500–1:1,000 | M** |
| Keratin 14 | LL002 | IgG3 | Novocastra | 1:25–1:100 | M |
| Keratin 15 | LHK15 | IgG2a | Neomarkers | 1:10–1:100 | M |
| Keratin 17 | E3 | IgG2b | DakoCytomation | 1:10–1:25 | M/F |
| Keratin 19 | A53-B/A2 | IgG2a | Abcam | 1:50–1:100 | M |
| Keratin 5 | XM26 | IgG1 | Novocastra | 1:250–1:500 | M/F |
| Keratin 5 | Rabbit | Covance | 1:1,000 | M | |
| Keratin 5/6 | D5/16B4 | IgG1 | Boehringer | 1:250 | M |
| Keratin 6a | Ks6.KA12 | IgG1 | Monosan | 1:10 | M/F |
| Keratin 7 | RCK105 | IgG1 | Abcam | 1:100 | M |
| Keratin 8 | LE41 | IgG1 | Lane, 1982 | 1:10 | M |
| Keratin 8 | 35βH11 | IgM | DakoCytomation | 1:25–1:100 | M |
| Ki-67 | MIB1 | IgG1 | DakoCytomation | 1:100 | M |
| Laminin-α1 | 161 EB7 | IgG | Maatta et al., 2001 | 1:5 | M |
| Laminin-α2 | 5H2 | IgG1 | Engvall et al., 1990 | 1:1 | M/F |
| MCM2 | CRCT2.1 | IgG1 | Abcam | 1:25–1:100 | F |
| MCSP | LHM2 | IgG1 | Abcam | 1:50 | M/F |
| MUC1 | 115D8 | IgG2b | Biogenesis | 1:10–1:50 | M** |
| Occludin | OC-3F10 | IgG1 | Zymed Laboratories | 1:50–1:100 | M |
| p63 | 7JUL | IgG1 | Novocastra | 1:10–1:25 | M |
| Smooth muscle actin | HHF35 | IgG1 | Enzo Diagnostics | 1:25 | M |
| SSEA-4 | MC-813-70 | IgG3 | Chemicon | 1:25–1:100 | M/F** |
| SSEA-4 | 5C1 | IgG3 (rat) | US Biological | 1:25–1:100 | M |
| Thy1 | AS02 | IgG1 | Dianova | 1:25–1:100 | M** |
| Vimentin | Vim3B4 | IgG2a | DakoCytomation | 1:25–1:100 | M |
| WT1 | 6F-H2 | IgG1 | DakoCytomation | 1:10–1:50 | M/F |
Staining was performed after fixation with methanol (M) and/or formaldehyde (F). Single asterisks indicate use in immunomagnetic cell sorting (MACS). Double asterisks indicate use in FACS analysis.