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The Ftr1p recycling signal is required for endocytic recycling of Fet3p. Yeast strains were constructed in which Fet3p-GFP was expressed in cells expressing full-length Ftr1p or Ftr1pΔ318 (top), or Ftr1p-GFP and Ftr1pΔ318-GFP in cells expressing a C-tail–truncated form of Fet3p, Fet3pΔ591 (bottom). The cells were grown in iron-deficient medium and imaged by fluorescence microscopy.
