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. 2007 Apr 9;177(1):173–182. doi: 10.1083/jcb.200612116

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Copyright © 2007, The Rockefeller University Press

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Figure 9. — Altered ceramide composition of 12R-LOX^−/− epidermis. Free and ester-linked epidermal lipids were extracted and separated using TLC. Ceramides are classified as suggested in previous works (Motta et al., 1993; Robson et al., 1994). B1–5 are lipids extracted after alkaline hydrolysis, most likely ester-linked epidermal lipids. (A) A representative TLC analysis of lipids extracted from wild-type and knockout epidermis. (B) Individual lipid levels quantified by densitometric analysis. Data are presented as the mean ± the SEM. n = 3. *, P < 0.01. Shown is one of two independent experiments with similar results.