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. 2007 Apr 23;177(2):205–210. doi: 10.1083/jcb.200607084

Figure 1.

Figure 1.

Overexpression of UNC-45FLAG results in decreased thick filament assembly and a mild paralysis phenotype. (A) Expression levels of UNC-45FLAG protein by two different integrated lines. Two integrated lines overexpressing UNC-45, suIs1, and suIs2 were analyzed for UNC-45FLAG levels by Western blots using a mAb to the C-terminal FLAG tag on transgenic UNC-45. Each lane contains 10 worms total. (B) UNC-45FLAG overexpression results in a mild paralysis phenotype. Motility in liquid was measured at 20°C for N2 and suIs2 grown at 20°C, unc-45(e286) grown at 15 and 20°C, and suIs2;unc-45(e286) grown at 25°C . N2 and suIs2 worms showed a mean of 33 and 21 body bends, respectively, over 15 s. The UNC-45FLAG transgene expressed in suIs2 was able to rescue the motility defect of unc-45(e286) at 25°C from a mean of 2 to 16 bends in 15 s. *, P < 0.001 versus N2 wild type. (C) suIs2 worms have decreased number of MHC-containing A bands in body wall muscle cells. Ya worms were labeled with an anti-MHC A mAb to reveal the presence of A bands. (D) Quantitation of the myosin assembly defect of suIs2 worms. The number of A bands per cell in regions I and II, posterior to the pharynx (Mackenzie et al., 1978b) of N2 and suIs2 worms, was counted. In region II, N2 worms have a mean of 7.8 A bands per cell, whereas susI2 worms have a mean of 5.8. In region III, N2 and suIs2 worms have 8.7 and 6.3 A bands per cell, respectively. *, P < 0.0001 versus N2 wild type. Error bars indicate SD.