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. 2007 Apr 23;177(2):211–218. doi: 10.1083/jcb.200701158

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — The upstream proline is required to confer CEA-like properties. (A) NCAM chimeras that were used to localize the CEA-specific signal. Mean FACS surface expression is indicated for LR and L6 transfectants. (B) The chimeric constructs were GPI anchored, as shown by cold Triton X-100 insolubility (top) and PIPLC sensitivity (bottom). (C) LR transfectants demonstrated that all proteins had the ability to mediate intercellular adhesion. (D) Binding by LR transfectants to immobilized Fn, in which values represent the mean ± SEM (error bars) for at least four independent experiments. (E) L6 cells showed similar effects on Fn binding. Values relative to parental cells represent the mean ± SEM for at least four independent experiments. (D and E) *, P < 0.0001. (F) Morphological differentiation of L6 transfectants. The fusion index, which is provided as a percentage, is given in the insets.