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. 2007 Apr 23;177(2):211–218. doi: 10.1083/jcb.200701158

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Scrambling the amino acid sequence in the identified key region. (A) Schematic of the constructs containing scrambled amino acids at positions 6–10, with the amino acid sequence shown underneath. Relative cell surface expression is shown. (B) GPI anchorage of these chimeric proteins was demonstrated by insolubility in cold Triton X-100 (top) and sensitivity to PIPLC treatment (bottom). (C) The scrambled constructs mediated intercellular adhesion in LR cells. (D) Binding to Fn by LR transfectants of the scrambled constructs. Values represent the mean ± SEM (error bars) for four independent experiments (*, P < 0.0005). (E) Effect of expression of the scrambled constructs on L6 Fn binding. Values represent the relative mean ± SEM for three independent experiments (*, P < 0.004; **, P < 0.03). (F) Morphological differentiation of L6 transfectants. The fusion index, which is provided as a percentage, is given in the insets. (G) Bio Biochemical differentiation in terms of myosin production as demonstrated by Western blotting on 5 μg of cellular lysate.