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. 2007 Apr 23;177(2):277–287. doi: 10.1083/jcb.200606065

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Four prosurvival proteins block tBid-induced permeabilization of isolated mitochondria. (A) Mitochondria from XEM or MLM were preincubated with recombinant prosurvival proteins at the indicated concentrations and supplemented with tBid for a further 2 h. Supernatant and pellets were examined by Western blotting for cytochrome c (in the MLM experiment, only half of the supernatant samples were loaded compared with the pellets). (B) C-terminal truncation of hBcl-x_L decreases potency. MLM were incubated as in A with full-length hBcl-x_L, a C-terminally truncated form (hBcl-x_LΔC24), or with Xenopus Bcl-x_LΔC23. (C) High concentrations of Bcl-w can complement Bcl-x_L–mediated protection. MLM were incubated as in A with Bcl-wΔC10, Bcl-x_L, or both before exposure to tBid at the indicated concentrations. S/N, supernatant; Mito, mitochondrial pellet.