Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jul 3;174(1):19–25. doi: 10.1083/jcb.200602049

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Loss of PFA4 causes Chs3 aggregation in the ER. (A) Cells expressing Chs3-3xHA and Chs7-GFP were subjected to immunoprecipitation with α-HA antiserum and analyzed by Western blotting with α-HA and α-GFP mAbs. Strains used were KLY46 (lanes 1 and 5), KLY45 + pTM15 (lanes 2 and 6), KLY46 + pTM15 (lanes 3 and 7), and KLY14 + pTM15 (lanes 4 and 8). (B) Lysates of wild-type, chs7Δ, and pfa4Δ cells transformed with plasmid-borne Chs3-3xHA (pHV7) were cross-linked using increasing concentrations of DSP; DTT was added to duplicate samples to reverse cross-links. Chs3 was detected by Western blotting with α-HA mAb. M, monomer; A, aggregates. (C) Cross-linking of chromosomally encoded Chs3-3xHA in wild-type (KLY41; ⋄), chs7Δ (KLY46; ▵), pfa4Δ (KLY43; □), and chs7Δ pfa4Δ (KLY45; ○) strains was performed as for B. Disappearance of monomeric Chs3 as a function of cross-linker concentration was quantified by densitometry.