Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Jul 3;174(1):53–63. doi: 10.1083/jcb.200604058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — ER-localized Golgi proteins up-regulate COPII assembly. (A and B) At various times of BFA treatment (A) and washout (B), methanol-fixed NRK cells were analyzed for COPII assembly and post-ER mannosidase II (manII; mean ± SEM; >15 cells each). COPII assembly increased upon BFA-induced ER localization of Golgi enzymes and returned to the steady-state level of untreated cells (dashed lines) upon Golgi enzyme exit. (C) COPII assembly was quantified in paraformaldehyde-fixed and Sec13-stained HeLa cells stably expressing T2-GFP. Control cells were those that expressed little or no detectable T2-GFP, whereas overexpressing cells yielded strong Golgi staining and detectable ER staining. Values are normalized means ± SEM (n = 4; >25 cells each).