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. 2006 Jul 3;174(1):53–63. doi: 10.1083/jcb.200604058

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Purified COPII is sufficient for increased assembly. (A–D) A permeabilized cell COPII assembly assay was performed on untreated (A and C) and BFA-treated (B and D) NRK cells. Reactions contained ATP and GTPγS and either the full COPII components Sar1p, Sec24p–Sec23p, and Sec13p–Sec31p (A and B) or just the inner COPII components Sar1p and Sec24p–Sec23p (C and D). Images are single optical sections of paraformaldehyde-fixed cells showing Sec24p localization. Bar, 10 μm. (E) Quantified results based on Sec24p staining of untreated and BFA-treated permeabilized NRK cells are compared for COPII assembly using the full COPII components Sar1p, Sec24p–Sec23p, and Sec13p–Sec31p or the inner COPII components Sar1p and Sec24p–Sec23p (normalized mean ± SEM; >15 cells each).