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. 2006 Jul 3;174(1):53–63. doi: 10.1083/jcb.200604058

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Binding of Sar1p to GalNAcT2 underlies assembly increase. (A and B) Permeabilized HeLa cells stably expressing GFP-T2 were incubated with GTPγS and 4 mg/ml cytosol containing the indicated concentration of R→A, the alanine-substituted peptide (A), or wt, the wild-type GalNAcT2 cytoplasmic domain peptide (B). COPII assembly, based on Sec13, was then quantified in T2-GFP–overexpressing cells and adjacent non- or low-expressing cells (mean ± SEM; >20 cells each). Representative images are presented in Fig. S2 (available at http://www.jcb.org/cgi/content/full/jcb.200604058/DC1). In contrast to the control, the GalNAcT2 peptide blocked the enhanced COPII assembly induced by GFP-T2 overexpression.