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. 2006 Jul 3;174(1):53–63. doi: 10.1083/jcb.200604058

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — VSVG up-regulates COPII assembly but not Golgi size. (A–F) Stable HeLa cell lines expressing VSVG-GFP were paraformaldehyde-fixed and analyzed to reveal GFP fluorescence (A and D) and Sec13 staining (B and E), each shown as Z axis projections of optical sections, as well as giantin staining (C and F) shown after 3D rendering. In contrast to control cells, VSVG-GFP–overexpressing cells yielded strong apparent ER and surface staining. Bar, 10 μm. (G) COPII assembly and Golgi size were quantified for control and overexpressing VSVG-GFP (G) cells. Values are normalized means (± SEM; n = 4; >25 cells each). Although COPII assembly increased in VSVG-GFP–expressing cells, Golgi size did not. (H) COPII assembly level and the Golgi/cell size ratio were also compared on a cell-by-cell basis for single experiments. The correlation plot yielded a slope of only 0.08.