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. 2006 Jul 3;174(1):89–100. doi: 10.1083/jcb.200604072

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Replicative age and colony-forming capacity as a function of replicative age. (a) The number of bud scars as an indicator of replicative age was assayed by calcofluor white M2R staining and flow cytometry. Shown is the fluorescence-intensity histogram with gates set to sort cells by number of bud scars. Gates used to sort cells by bud scar number are indicated by the bracketed lines. (b) Colony-forming capacity of cells separated by number of bud scars from SP cultures, quiescent, lower fractions, and nonquiescent, upper fractions in panel a. Cells from unfractionated exponential cultures (C) were used as a positive control for colony-forming capacity. Error bars indicate SD.