Table II.
Gene | Primer sequence | Strand | Product size | Reference |
---|---|---|---|---|
bp | ||||
Oligonucleotide primers used for quantitative real-time RT-PCR analysis of mouse genes |
||||
PAI-1 | 5′-GGGAAAAGGGGCTGTGTGAC-3′ | + | 406 | M33960 |
5′-GTACACGGTGTGTGGCTGTC-3′ | − | |||
Snail | 5′-ACCCCCGCCGGAAGCCCAACT-3′ | + | 127 | NM_011427 |
5′-AGCGGCGGGGTTGAGGACCTC-3′ | − | |||
Slug | 5′-CTCACCTCGGGAGCATACAGC-3′ | + | 146 | NM_011415 |
5′-TGAAGTGTCAGAGGAAGGCGGG-3′ | − | |||
Twist | 5′-CGGGTCATGGCTAACGTG-3′ | + | 196 | NM_011658 |
5′-CAGCTTGCCATCTTGGAGTC-3′ | − | |||
Hmga2 | 5′-AGCAAAAACAAGAGCCCCTCTA-3′ | + | 100 | NM_010441 |
5′-ACGACTTGTTGTGGCCATTTC-3′ | − | |||
Fibronectin-1 | 5′-CCCAGACTTATGGTGGCAATTC-3′ | + | 200 | NM_010233 |
5′-AATTTCCGCCTCGAGTCTGA-3′ | − | |||
Gapdh | 5′-TGTGTCCGTCGTGGATCTGA-3′ | + | 76 | NM_001001303 |
5′-CCTGCTTCACCACCTTCTTGA-3′ | − | |||
Oligonucleotide primers used for quantitative real-time RT-PCR in human cells |
||||
HMGA2 | 5′-CCCAAAGGCAGCAAAAACAA-3′ | + | 81 | NM_003483 |
5′-GCCTCTTGGCCGTTTTTCTC-3′ | − | |||
GAPDH | 5′-GGAGTCAACGGATTTGGTCGTA-3′ | + | 78 | BC023632 |
5′-GGCAACAATATCCACTTTACCAGAGT-3′ | − | |||
Oligonucleotide primers used to amplify different regions of the Hmga2 promoter |
||||
Hmga2 promoter −3420/−3320 | 5′-TAATGCGCTTGCCTGAGCTA-3′ | + | 100 | AC153362 |
5′-GCTGTCAAATCGGGCATCA-3′ | − | |||
Hmga2 promoter −495/−245 | 5′-TCCTGGCAGAAACTTCCACTCT-3′ | + | 250 | NM_010441 |
5′-TGGAGTGAATTGTGTCCCTTGA-3′ | − | |||
Hmga2 promoter −194/+4 | 5′-GAGCCTTTGCGGAGAGAGCAA-3′ | + | 200 | NM_010441 |
5′-CATCAACACCGGACGTCCA-3′ | − |