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. 2006 Jul 17;174(2):175–183. doi: 10.1083/jcb.200512110

Table II.

Oligonucleotide primers used for quantitative RT-PCR and ChIP

Gene Primer sequence Strand Product size Reference
bp
Oligonucleotide primers used for quantitative
    real-time RT-PCR analysis of mouse genes
     PAI-1 5′-GGGAAAAGGGGCTGTGTGAC-3′ + 406 M33960
5′-GTACACGGTGTGTGGCTGTC-3′
     Snail 5′-ACCCCCGCCGGAAGCCCAACT-3′ + 127 NM_011427
5′-AGCGGCGGGGTTGAGGACCTC-3′
     Slug 5′-CTCACCTCGGGAGCATACAGC-3′ + 146 NM_011415
5′-TGAAGTGTCAGAGGAAGGCGGG-3′
     Twist 5′-CGGGTCATGGCTAACGTG-3′ + 196 NM_011658
5′-CAGCTTGCCATCTTGGAGTC-3′
     Hmga2 5′-AGCAAAAACAAGAGCCCCTCTA-3′ + 100 NM_010441
5′-ACGACTTGTTGTGGCCATTTC-3′
     Fibronectin-1 5′-CCCAGACTTATGGTGGCAATTC-3′ + 200 NM_010233
5′-AATTTCCGCCTCGAGTCTGA-3′
     Gapdh 5′-TGTGTCCGTCGTGGATCTGA-3′ + 76 NM_001001303
5′-CCTGCTTCACCACCTTCTTGA-3′
Oligonucleotide primers used for quantitative
    real-time RT-PCR in human cells
     HMGA2 5′-CCCAAAGGCAGCAAAAACAA-3′ + 81 NM_003483
5′-GCCTCTTGGCCGTTTTTCTC-3′
     GAPDH 5′-GGAGTCAACGGATTTGGTCGTA-3′ + 78 BC023632
5′-GGCAACAATATCCACTTTACCAGAGT-3′
Oligonucleotide primers used to amplify
    different regions of the Hmga2 promoter
     Hmga2 promoter −3420/−3320 5′-TAATGCGCTTGCCTGAGCTA-3′ + 100 AC153362
5′-GCTGTCAAATCGGGCATCA-3′
     Hmga2 promoter −495/−245 5′-TCCTGGCAGAAACTTCCACTCT-3′ + 250 NM_010441
5′-TGGAGTGAATTGTGTCCCTTGA-3′
     Hmga2 promoter −194/+4 5′-GAGCCTTTGCGGAGAGAGCAA-3′ + 200 NM_010441
5′-CATCAACACCGGACGTCCA-3′