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. 2006 Jul 17;174(2):195–206. doi: 10.1083/jcb.200604022

Figure 1.

Figure 1.

DNA damage triggers the recruitment of condensin core subunits to damaged chromatin and the formation of UCC bodies. (A) Schematic of the condensin I and II complexes. H, G, and D2 denote condensin I non-SMC subunits hCAP-H, hCAP-G, and hCAP-D2, respectively. H2, G2, and D3 denote condensin II non-SMC subunits hCAP-H2, hCAP-G2, and hCAP-D3, respectively. (B) Recruitment of condensin proteins and UCC in HeLa cells after treatments with NCS. The cells were treated with 200 ng/ml NCS and immunostained 24 h later with anti-SMC2 antibody (red). DNA was counterstained with Yo-Pro-1 (green) or DAPI (blue). Bars, 8 μm. (C) Western blotting analysis of chromatin fractions obtained from untreated HeLa cells and 24 h after treatment with 200 ng/ml NCS, demonstrating recruitment of SMC2 and SMC4 to chromatin in response to the treatment. (D) Treatment of DNA-damaged cells with DNase-I leads to the dissociation of condensin proteins from DNA. Arrows indicate the nucleolar sites where UCC is formed. Arrows with asterisks in DNase-I–treated cells indicate nucleolar locations. Note the disappearance of condensin recruitment after DNase-I treatment. Bars, 20 μm.