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. 2006 Jul 17;174(2):195–206. doi: 10.1083/jcb.200604022

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Colocalization of UCC and coalesced nucleoli. (A) Simultaneous detection of the NCS-triggered recruitment of SMC2 (green) to UCC bodies and their colocalization with nucleoli marked by nucleostemin (NS) staining (red). DNA (DAPI) appears in blue. Note the nucleolar disruption after UV treatment versus nucleolar coalescence and colocalization of the resultant large nucleolar bodies with UCC bodies after NCS treatment. Bars, 20 μm. (B) Visualization of NCS-triggered UCC colocalized with coalesced nucleoli using immunostaining with an antinucleolin antibody. Nucleolin, green; SMC2, red; DNA (DAPI), blue. Bars, 8 μm.