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. 2007 May 21;177(4):599–611. doi: 10.1083/jcb.200609088

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — G2 arrest by the morphogenesis checkpoint does not depend on securin. (A–C) Strains with the indicated genotypes (ySP3575, ySP3435, and ySP3436) were grown at 25°C in YEPR. Elutriated G1 unbudded cells were released at 25°C in the presence of galactose (time 0), followed by FACS analysis of DNA contents (A) and determination of kinetics of budding, sister chromatid separation, spindle formation/elongation, and nuclear division (B). Micrographs (C) were taken at 240 min (wt), 420 min (4X GAL1-CLA4t), and 300 min (4X GAL1-CLA4t pds1Δ). (D–F) wt (ySP601), pds1Δ (ySP2894), and swe1Δ (ySP3887) cells were grown in YEPD at 23°C, arrested in G1 by α-factor, and released in the presence of Lat-A, followed by FACS analysis of DNA contents (D) and determination of kinetics of budding, sister chromatid separation, spindle formation/elongation, and nuclear division (E). (F) Micrographs represent cells at 90 min.