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. 2007 May 21;177(4):599–611. doi: 10.1083/jcb.200609088

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Cdc14 is not required for nuclear division of cdc55Δ cells with morphogenetic defects. (A) Wild-type (wt; ySP3575) and cdc55Δ (ySP5068) cells were arrested in G1 by α-factor at 30°C and released at 25°C in the presence of Lat-B (time 0). At the indicated times, cells were collected to determine the kinetics of budding, sister chromatid separation, nuclear division, and Cdc14 nucleolar export after in situ immunofluorescence. Micrographs (B) show examples of cdc55Δ cells at 150 min after release. (C) cdc24 cdc55Δ (ySP6503) and cdc24 cdc55Δ cdc14-3 (ySP6499) cells expressing myc-tagged Swi5 were arrested in G1 by α-factor at 25°C and released at 37°C (time 0). Cells were analyzed at the indicated times for budding, spindle formation/elongation, nuclear division, and Swi5 nuclear import. Pictures were taken at 150 min after release. (D) Strains with the indicated genotypes (ySP5704, ySP5710, and ySP5893) were grown in YEPR at 30°C, arrested in G1 by α-factor, and released in YEPRG at 25°C (time 0), followed by scoring GFP dots to determine the kinetics of sister chromatid separation.